Investigating the effect of sucrose concentration on conversion to glucose and fructose using InvertasePrediction==========I would expect to find that an increase of sucrose concentration would reduce the time it takes for clinistix to turn blue from red, or in other words, the reaction rate would increase with an increase in substrate concentration up to a certain point where the maximum reaction rate will be reached. A higher concentration of sucrose has more molecules inside Therefore a collision is more likely to occur, the molecules have to collide in order to react. This means that a reaction is more likely to occur in a shorter time, accelerating the reaction rate until the maximum reaction rate is reached, where an increase in substrate concentration would not increase the reaction rate because at this point all sites active are full. (1) Analyzing the Evidence Looking at my graph showing the average time taken (s) for clinistix to turn blue versus sucrose concentration (%), I can say that the general trend seems to be that as the sucrose concentration increases, the average time taken for clinistix turns blue and decreases. This is shown in my graph by a linear relationship going downwards (negative correlation). But for a more detailed interpretation I will focus on my second graph, which shows the reaction rate versus sucrose concentration: between 0.5% and 1%, there is a large increase in reaction rate, since the concentration doubles. There are more substrate molecules and therefore there is a greater chance of the substrate molecules bumping into......the center of the paper......the reason for my uncertainty may have been because I used a clinistix different from those I have used before. I do not consider the 4% result to be a major anomaly, as it did not influence the average results. References: 1) Www.Worthington-biochem.com/introBiochem/substrateConc.html2) Www.ntri.tamuk.eduk/cellular/enzyme2.html3) Janet L. Hopson and Norman K. Wessells (1990)Essentials of Biology- International Edition4 ) Ann Fullick (1994), Biology (Heinemann Advanced Science).Heinemann Educational Publishers (Oxford) Ltd.5) Www. midtermpapers.com/views/Science/.shtml6) NPO Green et al, (1990) Life Sciences-Organisms, Energy and the Environment, Second Edition. Published by the University of Cambridge Press Syndicate.7) Www.enzymes.co.uk/answer 45_invertase.htm