STUDY DESIGN OF MATERIALS AND METHODOLOGIES This was a comparative cross-sectional study that examined socioeconomic and diabetes-related factors such as exposure variables and Pseudomonas aeruginosa as an outcome variable. The study attempted to compare these variables in different private laboratories frequented by patients for sugar and crop testing in Sankari and Edappadi, Salem district, Tamil Nadu, India and was conducted from June 2013 to September 2013. TECHNIQUE SAMPLING AND SAMPLE SIZE The data obtained by conducting interviews, pus tests and blood samples of the respondents were determined (questionnaire in Annexure-1). The diabetic foot ulcer sample size was 77 for isolation of Pseudomonas aeruginosa from foot ulcer. VARIABLES1. Outcome variable (dependent): includes the occurrence of Pseudomonas aeruginosa from foot ulcer in diabetic patients at the time of the investigation. Explanatory variable (independent)1. Socioeconomic status: includes age category, gender, literacy status and economic status (annual income)2. Diabetes-related factors include duration of diabetes mellitus, type of treatment for diabetes mellitus at the time of the questionnaire, random blood glucose at the time of the questionnaire, disease associated with a foot infection, history of previous amputation, and maintenance of foot care.DATA MANAGEMENT AND ANALYSIS Data entry was performed using the IBM SPSS version-20 and MedCalc version 3 statistical package. Frequencies and cross tabulations were used to check for missing values ​​and variables. The strength of association between variables was determined using the odds ratio, with a 95% confidence level. SPECIMEN COLLECTION Foot ulcer specimens were collected from patients with diabetes at...... middle of paper ......tion of disc production. In this study, 14 antibiotics were used. Everyone belongs to different groups. Some of the standard antibiotics are: Amikacin (10 mcg), Amoxyclav (30 mcg), Ampicillin/Cloxacillin (10 mcg), Cefdinir (5 mcg), Cefixime (5 mcg), Cefotaxime (30 mcg), Ceftazidime (30 mcg), Cefuroxime (30 mcg), Cefalexin (30 mcg), Cetriaxone (30 mcg), Colistin (10 mcg), Co-trimoxazole (25 mcg), Gentamicin (30 mcg), Nalidixic acid (30 mcg), Norflexin (10 mcg) , Rossithromycin (30 mcg) and Streptomycin (10 mcg). The essential oils were eucalyptus oil, lemongrass oil, windergreen oil and peppermint oil. COMPOSITION OF MUELLER-HINTON AGAR Beef infusion: 300.0 g Acid hydrolyzate of casein: 17.5 g Agar: 17.0 g Starch: 1.5 g The pH was adjusted to 7.3 ± 0.2 a 25°C using a solution of 0.1 N hydrochloric acid (HCl) or 0.1 N sodium hydroxide (NaOH)